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benchling s crispr analysis tool  (Benchling Inc)

 
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    Benchling Inc benchling s crispr analysis tool
    Fig. 3. A representation of the outputs from the ICE (Inference of <t>CRISPR</t> Edits) software for a guide RNA targeting the slc45a2 gene in an Atlantic cod larva. (A) Sanger sequencing revealed indels in the Atlantic cod larva (ID = A1) treated with Cas9 mRNA-sg1–1. The target region is shown in green and the PAM site in yellow. (B) The inferred distribution and percentage of indels within the target region. (C) Discordance between the edited (in green) and control (in orange) trace files, illustrating the location and prevalence of editing. (D) Sanger sequencing traces after PCR of both edited and wild-type (control) Atlantic cod larvae in the region surrounding the guide sequence. The guide sequence is highlighted with a black underline, the PAM site with a red dotted underline, and the expected cut site is indicated by a vertical dotted line. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Benchling S Crispr Analysis Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/benchling s crispr analysis tool/product/Benchling Inc
    Average 86 stars, based on 1 article reviews
    benchling s crispr analysis tool - by Bioz Stars, 2026-06
    86/100 stars

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    1) Product Images from "CRISPR-Cas9/Cas12a-based genome editing in Atlantic cod (Gadus morhua)"

    Article Title: CRISPR-Cas9/Cas12a-based genome editing in Atlantic cod (Gadus morhua)

    Journal: Aquaculture

    doi: 10.1016/j.aquaculture.2023.740440

    Fig. 3. A representation of the outputs from the ICE (Inference of CRISPR Edits) software for a guide RNA targeting the slc45a2 gene in an Atlantic cod larva. (A) Sanger sequencing revealed indels in the Atlantic cod larva (ID = A1) treated with Cas9 mRNA-sg1–1. The target region is shown in green and the PAM site in yellow. (B) The inferred distribution and percentage of indels within the target region. (C) Discordance between the edited (in green) and control (in orange) trace files, illustrating the location and prevalence of editing. (D) Sanger sequencing traces after PCR of both edited and wild-type (control) Atlantic cod larvae in the region surrounding the guide sequence. The guide sequence is highlighted with a black underline, the PAM site with a red dotted underline, and the expected cut site is indicated by a vertical dotted line. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Fig. 3. A representation of the outputs from the ICE (Inference of CRISPR Edits) software for a guide RNA targeting the slc45a2 gene in an Atlantic cod larva. (A) Sanger sequencing revealed indels in the Atlantic cod larva (ID = A1) treated with Cas9 mRNA-sg1–1. The target region is shown in green and the PAM site in yellow. (B) The inferred distribution and percentage of indels within the target region. (C) Discordance between the edited (in green) and control (in orange) trace files, illustrating the location and prevalence of editing. (D) Sanger sequencing traces after PCR of both edited and wild-type (control) Atlantic cod larvae in the region surrounding the guide sequence. The guide sequence is highlighted with a black underline, the PAM site with a red dotted underline, and the expected cut site is indicated by a vertical dotted line. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: CRISPR, Software, Sequencing, Control



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    benchling s crispr analysis tool  (Benchling Inc)
    86
    Benchling Inc benchling s crispr analysis tool
    Fig. 3. A representation of the outputs from the ICE (Inference of <t>CRISPR</t> Edits) software for a guide RNA targeting the slc45a2 gene in an Atlantic cod larva. (A) Sanger sequencing revealed indels in the Atlantic cod larva (ID = A1) treated with Cas9 mRNA-sg1–1. The target region is shown in green and the PAM site in yellow. (B) The inferred distribution and percentage of indels within the target region. (C) Discordance between the edited (in green) and control (in orange) trace files, illustrating the location and prevalence of editing. (D) Sanger sequencing traces after PCR of both edited and wild-type (control) Atlantic cod larvae in the region surrounding the guide sequence. The guide sequence is highlighted with a black underline, the PAM site with a red dotted underline, and the expected cut site is indicated by a vertical dotted line. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Benchling S Crispr Analysis Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/benchling s crispr analysis tool/product/Benchling Inc
    Average 86 stars, based on 1 article reviews
    benchling s crispr analysis tool - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier

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    Fig. 3. A representation of the outputs from the ICE (Inference of CRISPR Edits) software for a guide RNA targeting the slc45a2 gene in an Atlantic cod larva. (A) Sanger sequencing revealed indels in the Atlantic cod larva (ID = A1) treated with Cas9 mRNA-sg1–1. The target region is shown in green and the PAM site in yellow. (B) The inferred distribution and percentage of indels within the target region. (C) Discordance between the edited (in green) and control (in orange) trace files, illustrating the location and prevalence of editing. (D) Sanger sequencing traces after PCR of both edited and wild-type (control) Atlantic cod larvae in the region surrounding the guide sequence. The guide sequence is highlighted with a black underline, the PAM site with a red dotted underline, and the expected cut site is indicated by a vertical dotted line. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Aquaculture

    Article Title: CRISPR-Cas9/Cas12a-based genome editing in Atlantic cod (Gadus morhua)

    doi: 10.1016/j.aquaculture.2023.740440

    Figure Lengend Snippet: Fig. 3. A representation of the outputs from the ICE (Inference of CRISPR Edits) software for a guide RNA targeting the slc45a2 gene in an Atlantic cod larva. (A) Sanger sequencing revealed indels in the Atlantic cod larva (ID = A1) treated with Cas9 mRNA-sg1–1. The target region is shown in green and the PAM site in yellow. (B) The inferred distribution and percentage of indels within the target region. (C) Discordance between the edited (in green) and control (in orange) trace files, illustrating the location and prevalence of editing. (D) Sanger sequencing traces after PCR of both edited and wild-type (control) Atlantic cod larvae in the region surrounding the guide sequence. The guide sequence is highlighted with a black underline, the PAM site with a red dotted underline, and the expected cut site is indicated by a vertical dotted line. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Four gRNAs were designed and selected with the webbased Benchling's CRISPR analysis tool (https://benchling.com) and CRISPOR tool (http://crispor.org; Concordet and Haeussler, 2018) with the gadMor3.0 Atlantic cod genome as template.

    Techniques: CRISPR, Software, Sequencing, Control